VBiolabs

Peptide Library Screening

Peptide libraries are useful resources to examine molecular interactions with small fragments of protein building blocks. Screening of peptide libraries provides a unique insight into epitope mapping of immune reactions. VBiolabs offer a construction and screening service of synthesized peptide libraries and phage display peptide libraries.

 

The following pre-made phage display random peptide libraries are ready for screening services.

 

Screening targets

Proteins, peptides, cells, specific surface and chemicals

 

Ph.D.™-7 Phage Display Peptide Library

Random heptapeptides are fused to a minor coat protein (pIII) of M13 phage. The displayed peptide (7-mer) is expressed at the N-terminus of pIII, starting from the first residue of the mature protein. The peptide is followed by a short spacer peptide (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence.
The number of primary clones: 2.8 x 109

 

Ph.D.™-12 Phage Display Peptide Library

Random dodecapeptides are fused to a minor coat protein (pIII) of M13 phage. The displayed peptide (12-mer) is expressed at the N-terminus of pIII, starting from the first residue of the mature protein. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence.
The number of primary clones: 2.7 x 109

 

Ph.D.™-C7C Phage Display Peptide Library

Random heptapeptides are fused to a minor coat protein (pIII) of M13 phage. The randomized sequence is flanked by a pair of cysteine residues, which spontaneously form a disulfide cross-link under nonreducing conditions, resulting in phage display of cyclized peptides. The disulfide-constrained heptapeptides are expressed at the N-terminus of plll, with the first cysteine preceded by an alanine residue and the second cysteine followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type plll sequence.
The number of primary clones: 1.2 x 109

 

PDL002 – Phage Display Peptide Library fd-PIII-6mer

Random 6-residue peptides are fused onto all 5 copies of a recombinant version of phage adsorption protein pIII and displayed on filamentous phage surface. The random amino acids are specified by degenerate codons in a synthetic oligonucleotide insert into recombinant gene rIII. The fused gene rIII is constitutively expressed and does not need to be induced.
The number of primary clones: 2.0 × 108

PDL003 - Phage Display Peptide Library fd-pIII-15mer

Random 15-residue peptides are fused onto all 5 copies of a recombinant version of phage adsorption protein pIII and displayed on filamentous phage surface. The random amino acids are specified by degenerate codons in a synthetic oligonucleotide insert into recombinant gene rIII. The fused gene rIII is constitutively expressed and does need to be induced.
The number of primary clones: 2.5 × 108
            

PDL004 - Phage Display Peptide Library fd-pVIII-15mer

Random 15-residue peptides are fused onto a recombinant version of phage protein pVIII and displayed on filamentous phage surface. The random amino acids are specified by degenerate codons in a synthetic oligonucleotide insert. The expression of fused gene rVIII is inducible by IPTG. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-15mer polypeptides derive from the recombinant, insert-bearing rVIII-15mer gene under fully induced conditions (1 mM IPTG) .
The number of primary clones: 2 × 109

PDL005 - Phage Display Peptide Library fd-pVIII-Cys0

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 0 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome contains the recombinant gene rVIII-Cys0 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys0 polypeptides derive from the recombinant, insert-bearing rVIII-Cys0 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 5.6 × 108

PDL006 - Phage Display Peptide Library fd-pVIII-Cys1

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 1 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome also contains the recombinant gene rVIII-Cys1 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys1 polypeptides derive from the recombinant, insert-bearing rVIII-Cys1 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 2.8 × 109


PDL007 - Phage Display Peptide Library fd-pVIII-Cys3

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 3 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome contains the recombinant gene rVIII-Cys3 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys3 polypeptides derive from the recombinant, insert-bearing rVIII-Cys3 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 5.2 × 107


PDL008 - Phage Display Peptide Library fd-pVIII-Cys4

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 4 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome contains the recombinant gene rVIII-Cys4 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys4 polypeptides derive from the recombinant, insert-bearing rVIII-Cys4 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 1.7 × 108


PDL009 - Phage Display Peptide Library fd-pVIII-Cys5

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 5 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome contains the recombinant gene rVIII-Cys5 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys5 polypeptides derive from the recombinant, insert-bearing rVIII-Cys5 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 5.9 × 108

PDL010 - Phage Display Peptide Library fd-pVIII-Cys6

Random peptides are fused to a recombinant version of the major coat protein pVIII and displayed on filamentous phage surface. Each displayed peptide has two cysteines at fixed positions 6 residues apart within the otherwise randomized amino acids, and thus is potentially constrained conformationally by a disulfide bond. The phage genome contains the recombinant gene rVIII-Cys6 and the wild-type gene VIII, which is expressed constitutively. Most of the 4000 major coat-protein subunits in the virion derive from the wild-type VIII gene, but up to 300 subunits are rpVIII-Cys6 polypeptides derive from the recombinant, insert-bearing rVIII-Cys6 gene under fully induced conditions (1 mM IPTG).
The number of primary clones: 2.7 × 108
 

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