VBiolabs

Phage Display

VBiolabs develops and screens various custom phage display libraries against academic, therapeutic and diagnostic targets for biomedical and pharmaceutical clients. Our phage display libraries include scFv/Fab antibody libraries, peptide libraries and cDNA libraries. We also construct camelised human single domain antibody libraries for our customers. The connection between genotype and phenotype enables large libraries of proteins or peptides to be screened and amplified in a process of in vitro selection, simulating the natural selection process. In addition, we can express small antibodies in prokaryotic systems and produce full-size humanized IgG antibody in genetic-modified CHO/293 cells.

With a range of technical systems available, our technical experts will assist you in selecting the right vector and screening strategy. VBiolabs also work together with our clients to design, execute and analyse the library and the screening. Our experts are highly experienced thus are able to help customers with a rapid and effective service. To discuss your specific requirements please send an email to This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Pre-made phage display peptide libraries are available for screening service

Types of bacteriophage

M13, Fd, F1, T4, T7 and λ phage

Types of phage display systems

True phage vector system
Phagemid system

Types of phage display libraries

Antibody phage display library:

                 Immunized antibody libraries
                 Naive antibody libraries
                 scFv/Fab antibody libraries
                 Semi-synthetic/synthetic antibody libraries

Screening targets

Proteins, peptides, cells, specific surface and chemicals

Applications of phage display

Antibody screening

A phage library expressing the appropriate parts of antibody molecules is screened for those that bind to a target antigen

Antibody Humanisation

Functional human-like antibodies could be generated from rodent or other antibodies

Peptide Screening

A random peptide library is displayed on the surface of bacteriophage and screened for specific binding with target molecules

Protein interaction
Screen of a large collection of proteins or peptides for binding to selected targets

Introduction to Phage Display Technology

The DNA encoding the protein/peptide of interest is fused with a gene encoding the protein that forms the viral coat of bacteriophage. When replicating, this virus produces phage particles that display the hybrid protein on the outside of their coats. The phage with the specifically displayed protein/peptide can then be used to fish for binding partners in a large pool of potential target proteins. Alternatively, a large pool of proteins/peptides displayed on phage particles can be screened for binding to a particular target molecule or a collection of molecules.