VBiolabs

Roche 454 GS FLX System Using Titanium Chemistry

VBiolabs enables high quality sequencing service using the Roche 454 Genome Sequencer FLX System (GS FLX). Roche 454 GS FLX is a next-generation DNA sequencing technology featuring a unique mix of relatively long reads (400-500 bp), exceptional accuracy, and ultra-high throughput (1 million reads/run). It has been proven to be a versatile sequencing technology based on many high-profile studies in several applications. Roche 454 GS FLX users have pursued innovative research in de novo sequencing, re-sequencing of whole genomes and target DNA regions, metagenomics, and RNA analysis.

Recent achievements of this powerful tool include sequencing the genome of an individual human and the detection of low-frequency somatic mutations linked to cancer.

 

Main application

Shotgun sequencing
De Novo Genome Sequencing
Large construct sequencing
CHIP-Seq
SNP screening and validation
Primer walking

Fragment analysis

Microsatellite analysis
Amplified fragment length polymorphism (AFLP)
Loss of heterozygosity (LOH)
Single strand conformation polymorphism (SSCP)

RNA sequencing

Serial Analysis of Gene Expression (SAGE) sequencing
Full-length cDNA analysis
Noncoding RNA Sequencing
MicroRNA

Technical features

Read-length: 400 bp/read
Number of reads/run: 1 million reads/run
Samples/run: 16 regions (1 plate)
 

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